Evaluation of PCL-PANi-GEL Nanofibrous Scaffolds for Tissue Engineering Purposes

Background and Aim: The structure of biocompatible scaffolds is one ofthe biggest concerns of tissue engineers. Ideally, a scaffold should mimicstructural and biological functions of the extracellular matrix (ECM);both in terms of physical structure and chemical component. Finding afeasible combination of polymers to fabricate a feasible scaffold is a bigconcern of scientists, therefore, we aim for finding a proper setup for auseful scaffold for tissue engineering purposes.Methods: Nanofibers were fabricated using electrospinning and to definetheir physical structure SEM imaging was used to evaluate the fibers abilityand potential in tissue engineering MTT assay was used furthermore thefibers ability to help stem cell differentiation was evaluated by osteogenicdifferentiation of hMSCs. Results: Our results showed that there can be a fine composition to helpstem cell differentiation using PCL PANi GEL not only we observed thatthis nanofiber is able to support stem cell differentiation but it can alsohelp stem cell proliferation and cell attachment.Conclusion: we concluded that PCL PANi GEL is a feasible setup forsupporting stem cell differentiation and proliferation also it can beconcluded that this combination of polymers is able to help support stemcells for different purposes of tissue engineering.

کلیدواژه ها:

Nanofibers ، Nano-scaffold ، Stem cell differentiation