Synthesis and Evaluation of Nanocomposite Scaffolds Based on Polyurethane and its Effect on Mesenchymal Stem Cells Proliferation

Background and Aim: Polyurethane (PU) is a biodegradable andbiocompatible polymer with various medical applications which isproper for use in the proliferation of stem cells. Different nanoparticlescan improve the structural characteristics of scaffolds for adhesive,migration and proliferation of cell lines such as Au, Fe, Zn and cobalt nanoparticles (CoNPs). The aim of this study is designing a new andproper scaffold to evaluate the stem cells adherence and proliferationMethods: PU scaffolds and CoNPs were characterized by followingmethods and techniques: the size of CoNPs was reported by Zeta Sizer(DLS), and scaffold properties were evaluated by Fourier transform infraredspectroscopy (FTIR), contact angle, scanning electron microscopy (SEM)and differential scanning colorimetric (DSC). MTT assay and AcridineOrange/Ethidium Bromide (AO/EB) staining were used to assess thebiocompatibility of CoNPs and PU scaffolds regarding cell attachmentand proliferation support.Results: DLS technique showed the average range of 30 nm for CoNPs.FTIR results showed the presence of CoNPs inside the PU nanocompositescaffolds. The contact angle results indicated that the surface hydrophilicityof the PU-CoNPs was increased after adding CoNPs to PUs. SEM imagesshowed that the cells were adhered, migrated and proliferated well onthe scaffolds. Acridine Orange staining and MTT assay showed that 1 mg/mL of CoNPs and 1% PU-CoNPs are not only toxic to the cells but alsoincrease their proliferation. Our results showed that CoNPs/PU is highlyeffective, biodegradable and biocompatible enough to apply in MSCsproliferation.Conclusion: According to the results, PU-CoNPs could be an appropriatescaffold for supporting cell adherence and proliferation as well asosteogenic differentiation of stem cells.

کلیدواژه ها:

Nanocomposite Scaffold ، Cobalt nanoparticles ، Polyurethane ، Stem Cells Proliferation