Evaluation of Electrospun Biodegradable Polycaprolactone and Hyaluronic Acid Nanofibers Containing Vitamin C for Tissue Engineering

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 381

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شناسه ملی سند علمی:

NSCMRMED03_253

تاریخ نمایه سازی: 30 دی 1397

چکیده مقاله:

Background and Aim: Electrospinning technique is one of the mostpromising methods for scaffold fabrication to micro and nanoscale intissue engineering. Hyaluronic acid is a biocompatible polysaccharidewhich is the main component of the extracellular matrix. Vitamin Cis a principal antioxidant in human blood which plays a crucial rolein the synthesis of collagen. In this study, polycaprolactone andpolycaprolactone/ hyaluronic acid/ vitamin C nanofiber scaffolds werefabricated through electrospinning technique and L929 fibroblast cellsseeded on nanofibers scaffolds.Methods: Polycaprolactone (PCL) and polycaprolactone (PCL)/hyaluronic acid (HA)/ vitamin C with weight ratios of (100:0), (90:10),(85:15), (80:20) and 40 mg vitamin C were dissolved in formic acid andacetic acid (70:30). The solution was electrospun with the flow rate of0.1 mL/h, with an applied voltage of 15 KV and 10 cm spinning distance.L929 fibroblast cells were seeded on nanofiber scaffolds to evaluate theproliferation ability with an MTT assay. SEM was performed on L929fibroblast cells cultured on PCL and PCL/ HA/ vitamin C.Results: The SEM images showed higher cell growth, proliferation andadhesion of L929 fibroblast cells on the PCL/ HA/ vitamin C scaffoldscompared to the PCL scaffold. SEM results showed that adhesion offibroblast cells increased with increasing the concentration of hyaluronicacid. The results of the MTT assay showed PCL and PCL/ HA/ vitamin Cnanofiber scaffolds were non-toxic.Conclusion: The results showed that PCL/ HA/ vitamin C nanofiberscaffolds have a great potential for using in tissue engineering. In-vitro tests showed high cell adhesion and viability on the electrospun scaffolds.

نویسندگان

Mahsa Janmohammadi

Biomaterial group, Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran

Mohammad Sadegh Nourbakhsh

Biomaterial group, Faculty of New Sciences and Technologies, Semnan University, Semnan, Iran