Silk Nanofibrous Electrospun Scaffold Enhances Differentiation of Induced Pluripotent Stem Cells Into Insulin-Producing Cells

Background and Aim: The scaffolds accompanied with stem cellshave great potential for applications in pancreatic tissue engineering.Fabrication of nanofibrous scaffold similar to extracellular matrix isone of the applicable methods in pancreatic tissue regeneration. Theaim of this study was the fabrication of a silk nanofibrous scaffold asa microenvironment for pancreatic guiding differentiation of inducedpluripotent stem cells (iPSCs) toward beta-like cells.Methods: IPSCs were seeded on silk scaffolds and differentiated toinsulin-producing cells (IPC) using pancreatic medium supplementedwith growth factors for 21 days. The generation of IPC was confirmedvia the evaluation of specific pancreatic markers; insulin and pdx1 usingimmunocytochemistry and real-time PCR.Results: Our results in the MTT assay showed that silk scaffold supportsthe attachment and proliferation of iPSCs. The expression of pancreaticmarkers was higher in cells grown on silk scaffold in compare tomonolayer group.Conclusion: This study suggests electrospun silk nanofibrous scaffold asan appropriate substrate for pancreatic induction of stem cells that isapplicable for islet transplantation.

کلیدواژه ها:

Induced pluripotent stem cells ، Silk nanofibrous scaffold ، Insulin-producing cells ، Tissue engineering