Comparison of 2 Methods of Cell Culture on the Structure and Differentiation of Quail Bone Marrow Stem Cells

Background and Aim: Investigating, isolating and cultivating stemcells and studying animal models is one of the strategies for accuratelyunderstanding the functioning of these cells in the context of stem cellresearch. This study was conducted to determine the effect of isolationways and cell culture on differentiation and morphology of stem cells inmesenchymal cells of bone marrow in quails.Methods: For this purpose, 8 Japanese quails (free of the pathogen) wereraised. 1 mL of bone marrow at the age of six months was obtainedfrom a large bone marrow area and the fluid was transferred to DMEMmedium. Two groups were used for this study first group as direct withRBC and the second group RBC defected by Ficoll loading method. Theculture medium contained 10% bovine embryo serum with penicillin andstreptomycin antibiotics. Fourth passage cells were studied structurallyand differentiated into fat cells, bone marrow cells, and cartilage cells.The phenotypic measurement criterion in this study is the effect ofadipose, osteogenesis, and chondrogenesis of stem cell populations.Results: Based on the results, cloning in direct culture medium wascreated more than the loading medium on the Ficoll. In a direct culturemedium, more cells received the ability to differentiate into cartilage,bone and fat cells. In a direct culture, most of the cells had fibroblasticmorphology.Conclusion: In conclusion, direct culture media had better performancefor bone marrow mesenchymal cells.

کلیدواژه ها:

Quail ، Bone marrow stem cell ، Mesenchymal cells ، DMEM