Neural Proliferation and Differentiation of Human Adipose-Derived Stem Cells (hADSCs) On Nanofibers PCL/lignin Scaffold

Background and Aim: Tissue Engineering in regenerative medicinewarrants our enhanced understanding of the biomaterials and itsfunction. The aim of this study was the evaluation of the proliferation anddifferentiation of human adipose-derived stem cells (hADSCs) growingon Polycaprolactone PCL/lignin scaffold that we developed an aligned(PCL)/lignin nanofiber using electrospinning technique. Stability of thisscaffold is in providing a favorable matrix for cell growth.Methods: Following the isolation of hADSCs from human adipose tissue,these cells were cultured and characterized on PCL/LIGNIN scaffold.The MTT assay, lineage-specific differentiation under two-dimensionalculture conditions, scanning electron microscopy (SEM), flow cytometry,and immunocytochemistry were used for analyses.Results: The study revealed that the PCL/lignin fibers promote cellproliferation coupled with 90% cell viability and the excellent substratefor the differentiation of hADSCs to nerve cell.Conclusion: Our results suggest that PCL/lignin is a promising scaffold forneural proliferation and differentiation.

کلیدواژه ها:

Nervous system ، Stem cell ، Proliferation ، Differentiation ، Electrospinning ، Nanofibers