Platelet-Rich Plasma and its Restorative Effect on Fertility for Rat Model of Menopause; A Pre-cinical Study

Background and Aim: Due to natural physiological aging, women atage 50-52 years old, face menopause and its related obstacle includinginfertility. Early menopause occurs in about 1% of women under 40and 0.1% under 30 years in general population. Several causes havebeen identified for early menopause such as iatrogenic causes (mostlybecause of oncology treatment), idiopathic, genetic and autoimmunediseases. Several therapies have been proposed in this regard, however,the effectiveness of them remains to be further illustrated.Methods: In this study, we examined the restorative effect of Platelet-richplasma (PRP) as a natural blood product in the rat model of menopause.The animal models were made within 15 days, using a daily injectionof the gonadotoxic agent, 4-Vinylcyclohexene Diepoxide. The controlgroup received saline in the whole period of animal modeling. Afterconfirmation of menopause by hormonal (E2 and FSH) assessment andhistological studies, a group of rats was injected (intra-ovarian injection)by freshly prepared rat PRP in two concentrations of 1 and 4X (15 rat/group). Other two groups were either received saline or did not get anyinjection. Two months were assigned for evaluation of the treatment, inwhich every two weeks, 3 rats from each group randomly were allocatedfor hormonal and histological assessment of ovarian tissue.Results: Results indicate that the groups of rats who have receivedPRP in both concentrations restored somehow their ovarian function.Histological studies revealed that PRP treatment improved the folliclenumbers of treated rats compared to the non-treated ones. Hormonalassessment as well showed a refinement pattern as the level of FSH hasdeclined and the level of E2 has elevated significantly in the treatedcompared to non-treated rats.Conclusion: Our results illustrate that PRP could be applied as analternative therapeutic agent for treatment of patients who are at earlystages of menopause or about to enter into this complication such ascancer patients who are undergoing through gonadotoxic chemotherapyand radiotherapy. Quite optimistic, but further experimentations shouldbe performed to approve the safety and applicability of the technique inthe human setup.

کلیدواژه ها:

Menopause ، Infertility ، Platelet-Rich Plasma ، Animal Model ، Ovarian rejuvenation