Multi-Layered Cellular Biointerface for Bone Tissue Regeneration

Background and Aim: Orthopedic surgery is one of the key applicationsof tissue engineering. To date, numerous and various materials (syntheticand natural) are used. However, the optimization of their propertiesin order to improve bone tissue restoration is still challenging. Thisstudy aims to produce a tissue-engineered construct with tissue-likebiointerface using multi-layered cell sheets for bone regeneration.Methods: Scaffolds were fabricated via surface selective laser sintering ofpolylactide particles. Before structuring, polylactide particles were treatedwith hyaluronic acid. After sintering, structures were reinforced withIrgacure 2959-assisted photo-crosslinking. Mesenchymal stromal cells(MSCs) phenotype was determined using flow cytometry. Osteogenesiswas evaluated by measurement of calcium accumulation.Results: The scaffolds ensured successful adhesion and proliferationof human bone marrow MSCs and induced calcium deposition whichwas detected in 7-21 days of culturing in standard medium. The novelapproach to graft the scaffolds with a functional bio-interface wasproposed using surface functionalization with cell MSC sheets aiming tofacilitate host tissue-implant integration. The sheets exhibited contiguousmorphology and high viability.Conclusion: In this study, we presented a new approach to produceosteo-inducing constructs for bone-tissue regeneration grafted with multilayeredcellular bio-interface. The combination of cell sheet technologyand scaffolding can ensure the development of novel medical devices,which improve tissue reparation and regeneration. The achieved resultsform the base of new tissue engineering approach to fabricate osteosubstitutes.The study is supported by the Russian Science Foundationunder grant 18-15-00407.

کلیدواژه ها:

Tissue Engineering ، Osteogenesis ، Scaffolds