Production of GBM-Induced Rat Model: A Gateway to Preclinical Studies and Personalized Medicine for Human GlioblastomaMultiforme

Background and Aim: The glioblastoma multiforme (GBM) is anaggressive tumor in adults. It mostly develops in the brain, whereas, rarely spreads out of the central nervous system. It is the second most commonCNS malignancy in humans and is associated with high mortality rates.To establish a platform for drug discovery and preclinical studies, animalmodels of tumors is one of the most beneficial approaches. In this paper,we report our experience of developing a GBM-induced rat model.Methods: Using C6 cell line (malignant glial cell lines of Rattusnorvegicus),the cells were injected into the brain of Wistar rats. The rats started havinghemorrhagic discharge from their eyes and paraplegia on the medianof 6 days post-injection. Brain magnetic resonance imaging (MRI) wasperformed on the 8th-day post-injection to confirm the development ofthe tumor.Results: The model was established by employing the C6 cell line,which is immunologically compatible with its receptor. By exploitingbioinformatic tools, the similarities between aberrant gene expressionand pathways have been predicted. In this regard, 337 commensurablegenes and a number of pathways have been detected. We found genomicsimilarities between human- and rat-origin GBM. Moreover, comparingfilm outputs of MRIs from GBM-induced rats and 3 humans with GBM,we found close similarities. The pathologic analyses also showedcomparable results between human GBM samples and GBM samplesfrom sacrificed rats.Conclusion: Provision of cancer-induced models is a prerequisite toinvestigating not only the pathogenesis and pathology of tumors but alsoto improve drug discovery studies, preclinical research, and personalizedmedicine. Although several methods and various species have beenemployed for this approach, the real recapitulation of the human tumorhas been left under discussion. In this study, a GBM-induced rat modelwas established with comparable pathologic, genomic and radiologicfeatures to human-origin GBM.

کلیدواژه ها:

Animal Models ، Glioblastoma multiforme ، Rats