Conductive Drug-Loaded Electrospun Scaffold Based on Polyaniline for Nerve Tissue Engineering

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 346

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شناسه ملی سند علمی:

NSCMRMED03_112

تاریخ نمایه سازی: 30 دی 1397

چکیده مقاله:

Background and Aim: Bioelectricity, along with other vital signals, playsan important role in tissue engineering and regenerative medicine.The crucial step of entering this area is to design a suitable substratefor transmitting necessary signals to the cells. Conductive nanofibrousscaffolds are an appropriate tool for this purpose and have been highlyregarded due to their resemblance to the native extracellular matrix(ECM) and their ability to regulate cell behaviors. Herein, we aimed toproduce a drug-loaded conductive nanofibrous scaffold for nerve tissueengineering.Methods: Polyethersulfone (PES) nanofibers were prepared usingelectrospinning technique. Surface activation and enhancement ofsurface hydrophilicity were achieved by using oxygen plasma treatment.Freshly distilled aniline and ammonium persulfate (APS) were dissolvedseparately in an aqueous solution of the drug. Afterward, the PESelectrospun scaffold was fixed to plexiglass frame for the retention of itsoriginal form and was immersed in the drug/aniline solution at 40°C for a specified time. Chemical polymerization was then initiated by addingoxidant solution containing APS and conductive layer was polymerizedat around 5°C for 48 hours. In this way, the core/shell nanofibers weresynthesized via in situ chemical oxidative polymerization of anilineand the molecules of the drug were incorporated in polyaniline duringpolymerization. By adjusting the electrospinning parameters andthe polymerization conditions the desirable nanofiber structure wasfabricated. The PES/PANI/drug nanofibrous scaffold was characterizedusing scanning electron microscopy (SEM), attenuated total internalreflectance infrared spectroscopy (ATR-FTIR), electrical conductivity andcell culture study.Results: Based on SEM images, PES nanofibers prepared here werehomogenous and defect-free. As well as this, the conductive core/shellscaffold was coated uniformly. It was shown that plasma treatmenthad a significant effect on nanofibers coating process by increasinghydrophilicity and functionality of nanofibers surface. ATR-FTIR spectrafor PES and PANI/PES/drug nanofibers and FTIR spectra for powdermaterial of PANI confirmed the presence of PANI and drug on the surfaceof nanofibres. Additionally, the conductive scaffold offered acceptablevalue of conductivity. It was found that the conductivity and morphologyof the final nanofibers were strongly influenced by the dopant properties,the concentration of aniline and dopant, and time of polymerization.In addition, the viability of adipose-derived stem cells cultured on theconductive nanofibrous scaffold was found to be excellent and cells hadgood adhesion to the scaffold.Conclusion: Herein, we developed a drug-loaded conductive nanofibrousscaffold with a proper cell compatibility. By the combination ofnanofibrous topography, electrical activity, and biochemical functionalitynew multi-functional material with unique physicochemical propertieswas obtained. The natural conductivity of these composites offersexciting opportunities for electrical stimulation of cells, especially thoseare sensitive to electrical signals such as nerve cells. So, our scaffoldcan be an appropriate tool for regulating stem cells behaviors such asproliferation and differentiation.

نویسندگان

Zohreh DaraeiNejad

Biomedical Engineering Faculty, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran

Iman Shabani

Biomedical Engineering Faculty, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran

Ehsan Seyedjafari

Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran