Different Cytokines Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells

Background and Aim: During the initial stages of bone repair, somebiological phenomena emerge. Of these, proinflammatory cytokinesare released in the injury site, which quickly followed by a shift to antiinflammatorycytokines. However, the biological impacts of pro- and antiinflammatorycytokines on osteogenic differentiation of mesenchymalstem cells seems to be controversial. In this current investigation, westudied the effect of the proinflammatory cytokines (i.e., TNF-α, IL-6, IL-8,and IL-17F and the anti-inflammatory cytokine IL-4) on the proliferationand osteogenic differentiation of the human adipose stem cells (hASCs). Methods: In practice, the hASCs were treated with TNF-α, IL-6, IL-8, IL-17F, or IL-4 (10 ng/mL) for 72 hours mimicking bone repair.Results: Our findings revealed that the TNF-α could reduce the geneexpression of collagen type I, while it increased the proliferation ofhASC and the activity of ALP. IL-6 was found to significantly enhancethe activity of ALP (18-fold), while the bone nodule formation was alsoincreased by the hASCs. IL-8 did not affect the proliferation or osteogenicgene expression, however it lessened the bone nodule formation. IL-17Fwas found to decrease the proliferation of hASC, while it increased theactivity of ALP. IL-4 appeared to enhance the osteocalcin gene expressionand the activity of ALP; nonetheless, it decreased the gene expression ofRUNX2 and the bone nodule formation.Conclusion: Take all, our results showed that all cytokines studieddisplayed both inreasing and decreasing impacts on the osteogenicdifferentiation of hASCs, even when applied for 72 hours only. Somecytokines (i.e., IL-6) might be appropriate agents for the induction ofthe osteogenic differentiation of mesenchymal stem cells as a suitablestrategy for enhancing bone repair.

کلیدواژه ها:

Cytokines Osteogenic ، Differentiation ، Human adipose stem cells ، Bone repair