Differentiation of Human Adipose Stem Cells in Filament-Like Hydrogel Through Visible Light-Initiated Crosslinking

Background and Aim: Hydrogel fibers are structurally and biologicallyuseful devices for fabrication of filament-like tissues and differentiationof stem cells. We established cell-laden degradable hydrogel fibersthrough visible light-initiated crosslinking to differentiate stem cells andfabricate filament-like tissue.Methods: The human adipose stem cells (hADSCs) cell-laden fibers werefabricated by cross-linking phenolic-substituted alginate and gelatin (Alg-Ph and Gela-Ph respectively) in an aqueous solution containing cellsthrough irradiating visible light. The crosslinking of phenolic moietieswas mediated by ruthenium (II) tris-bipyridyldication (Ru (II) bpy andsodium ammonium persulfate (SPS).Results: The encapsulated hADSCs proliferated and grew withincell-laden hydrogel fiber. The encapsulated cells maintained theirpluripotency ability and could form filament-like tissue. The filament-liketissues covered with an additional heterogeneous cell layer was madeby degrading the fiber membrane using alginate-lyase after coveringthe fiber surface with vascular endothelial cells. The cellular viabilityis preserved during Alg-Ph and Gela-Ph hydrogel fiber fabrication andfilament-like tissue formation.Conclusion: These results demonstrate the feasibility of alginate-basedhydrogel fibers obtained through the Ru/SPS-mediated crosslinkingsystem and visible light irradiation for the engineering of filament-liketissues.

کلیدواژه ها:

Alginate and gelatin derivative hydrogel ، Fiber ، Visible light irradiation ، Filament-like tissues ، Biodegradation