Application of Multiplex Real Time PCR for Blood Born Infectious Diagnostics

Multiplex PCR is a modification of the original PCR procedure which allows the amplification of DNA from different pathogens independently and simultaneously by the use of several primers pairs in the same reaction. Multiplex PCR provides an additional advantage in screening since it detects multiple pathogens simultaneously in a single clinical sample with high sensitivity and specificity which proved to be a suitable method in the diagnosis of blood born infectious diagnostics. It is a cost effective diagnostic test because it reduces labor intensiveness, the economy of both test sample and reagents compared to single analytical PCRs, faster detection and also it simplifies downstream processing. Several studies have underscored the superiority of multiplex real time PCR in pathogen detection in terms of its sensitivity, specificity, reliability, timeliness as well as its advantages over the syndromic approach in blood born case detection and management. Thus, modern molecular diagnostic methodologies which provide a uniform platform for detecting both single and multiple pathogens simultaneously can be very useful in addressing blood born infections.

کلیدواژه ها:

Real Time PCR ، Blood born viruses ، Nucleic acid Amplification Technology (NAT)