ASSOCIATION OF NERVE CONDUCTION AND PLASMA HOMOCYSTEINE, FOLIC ACID AND VITAMIN B12 LEVELS WITH MTHRF GENOTYPES IN DIABETIC POLYNEUROPATHY PATIENTS

Background and Aim: The methylenetetrahydrofolate reductase C677T and A1298C mutations may influence on plasma homocysteine, vitamin B12 and folate levels and diabetic polyneuropathy (DPN) developmentMethods: We performed a cross-sectional study of 80 patients (mean age: 55.1±5.8y) in Isfahan, Iran. The aim of present cross-sectional study were to evaluate (1) the relationship between plasma homocysteine (Hcy), folic acid, B12 levels and MTHFR C677T and A1298C genotypes, (2) Sensory and motor Nerve Conduction Velocity(NCV) variations according to MTHFR C677T and A1298C genotypes.Results: Of the 80 patients selected, 44 (55%) carried the CC, 26(32.5%) the CT, and 10 (12.5%) the TT genotype. Patients with A1298C genotypes, 36 (45%) carried the AA, 36(45%) the AC, and 8 (10 %) the CC genotype. Plasma levels of folate (P <0.001) and vitamin B12 (P <0.001) between C677T genotypes were lower in the TT subjects than in the CC subjects. No significant differences in plasma homocysteine level between C677T genotypes were demonstrated. Patients with the AA genotype demonstrated significantly higher plasma Hcy levels (11.1±1.9 micmol/L), lower plasma levels of folate (7.2±1.5 ng/ml) and vitamin B12 (404.9±76.9 pg/ml) compared to others A1298C genotypes. It was demonstrated that motor peroneal nerve velocity between C677T and A1298C genotypes was higher in the TT (P=0.053) and the AA (P=0.019) patients compared with other genotypes, respectively. Although, other NCV indices were not related with MTHFR genotypes.Conclusion: The present study findings report that MTHFR gene polymorphisms may influence on plasma levels of homocysteine, folate, and vitamin B12 and risk of DPN

کلیدواژه ها:

Homocysteine ، Folate ، Vitamin B12 ، Diabetic polyneuropathy