Investigation of antibiotic resistant patterns and metallo- beta lactamse genes of Klebsiellapneumoniae isolated from Beesat hospital, Hamedan

Background: KlebsiellaPneumoniaeis one of the important gram-negative bacteria that can involve in health care association infections (HSI). This pathogen can cause different infectious diseases like: urinary tract infection (UTI), septicemia, wound infection and etc….Thenantibiotic resistant strains especially carbapenem resistant make complication in antibiotic therapy process. Different mechanisms involve in resistance to carbapenem in K. pneumonia and metallo beta lactamase production is one of the important enzymatic resistant mechanisms. This study aimed to investigate of antibiotic resistance patternsof K. pneumonia isolated from a teaching hospital in Hamedan. On the other hand VIM, IMP, NDM-1, SPM-1 was detected by PCR assay. Methods: In this cross sectional study, 89K. pneumoniae collected from different clinicalsamples like, blood, urine, wounds etc. during 6 months. Antibiotic susceptibility testing was done according to CLSI against; cefepime, cefazolin, cefotaxime, ceftazidime, ceftriaxone, imipenem, gentamicin, amikacin, ciprofloxacin and SXTafter isolates were identified by conventional biochemical and microbiological methods. DNA extraction was prepared by thermo kit from USA and VIM, IMP, NDM-1; SPM-1 genes were detected by PCR and gel electrophoresis. Results: 16 carbapenem resistant K. pneumoniae were collected according to antibiotic susceptibility testing.25% of these strains resistant to all tested antibiotics.66% just susceptible to the amikacin. 6% susceptible to gentamicin and amikacin.87/5% were positive for Oxa-48and NDM-1 just identified in one strains. VIM, IMP and SPM-1were defined in none of isolates.Conclusion: K. pneumoniae is one of the important gram-negative bacteria that involve in nosocomial infections. Moreover, antibiotic resistance strains can make serious health problems especially carbapenem resistantbacteria.Oxa-48 was the most prevalent detected carbapenamse gene. This gene carried on movement genetic element and can transfer to other bacteria rapidly. So, detection of Oxa-48 producing bacteria can be necessary in hospitals.

کلیدواژه ها:

carbapenemase ، K. pneumoniae ، Hamedan