The correlation between biofilms formation capabilitieswith their related genes and antibiotic resistance patterns in clinical and environmental isolates of Pseudomonas aeruginosa

Background: Pseudomonas aeruginosais an important nosocomial pathogen which its infection is life-threatening because of their antimicrobial resistance. The integron genes and biofilm formation of P. aeruginosahave a significant role in antibiotic resistance. This study aimed at evaluating the correlation between biofilms formation capabilities of clinical and environmental P. aeruginosaisolates with their related genes. Methods: This cross-sectional study during 2017 was performed on 58 clinical and 20 environmental isolates of P. aeruginosa with collecting the 547 samples (439 of clinical and 108 of environmental samples). The isolates were identified by phenotypic and genotypic tests. Kirby-Bauer agar disk diffusion method was used for susceptibility testing. The prevalence of class I, II and III Integrons and rhlA and lasB genes was determined by PCR. Determining of biofilm formation was performed using a microtiter plate method. The Stata software was used for statistical analysis. Results: In total, the most prevalent of resistance was observed against Ticarcillin/Clavulanic Acid (55%). Generally, 44 (56.4%) of strains were producers of strong biofilm in both environmental and clinical isolates. The prevalence of strong biofilms producers in clinical isolates was more than environmental isolates. Also, a significant correlation was observed between IntI, IntII and rhlA genes with biofilm formation capability of isolates (P=0.02). Conclusion: Regarding more than fifty percent of both environmental and clinical isolates were producers of strong biofilms and because the source of clinical isolates may be from the environment, the necessary hygiene measurements should be performed for prevent of transferring the environmental isolates to hospitalized patients.

کلیدواژه ها:

Biofilm ، integron ، rhlA ، lasB ، Pseudomonas aeruginos