Vancomycin and linezolid resistance (van A, B, C and cfr) genes among Staphylococcus aureus clinical isolates

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 479

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شناسه ملی سند علمی:

MBMED05_018

تاریخ نمایه سازی: 1 دی 1397

چکیده مقاله:

Background: Increasing the resistance to methicillin and the ineffectiveness of β-lactams among methicillin-resistant S. aureus (MRSA) has led vancomycin and linezoli to be selective drugs for the treatment of Staphylococcal infections, in particular MRSA. The aim of this study was to investigate the vancomycin and linezolid resistance in clinical isolates of S. aureus. Methods: Two-hundred and seventy S. aureus isolates were identified with standard microbiology methods and were confirmed by femA gene. Antimicrobial susceptibility was performed by standard disk diffusion method ac cording to the Clinical and Laboratory Standards Institute (CLSI) guidelines. Vancomycin Resistance detection by agar dilution method and confirmed by E-test. The extraction of total genomic DNA were achieved by commercial extraction kits and PCR assay and sequence was further performed for detecting of mecA, van A, B and C and cfr gene by using specific primers. Results: One-hundred and fifty-two (56.3%) isolates were resistant to methicillin (MRSA) and 144 (53.3%) of isolates carried mecA gene. Six (1.2%) of isolates were non-susceptible to vancomycin and likewise 10 (3.7%) isolates were resistant to linezolid. Moreover, 2 (0.7%) of isolates carried vanA gene. The cfr gene not detected in any isolates. Conclusion: Resistance to methicillin was high and vancomycin resistance via vanA gene was detected which can increase the risk of the spread of vancomycin resistance. However, resistance to linezolid was not associated with the cfr gene. Therefore, linezolid is yet a valuable antimicrobial in the eradication of S. aureus infections.

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نویسندگان

Masoumeh Aslanimehr

Cellular and Molecular Research Center/ Medical Microbiology research center Qazvin University of Medical Sciences, Qazvin, Iran

Homa Kalhor

Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

Ameneh Barikani

Department of Social Health, Qazvin University of Medical Sciences, Qazvin, Iran

Amir Peymani

Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran