Characterization by phenotypic and genotypic methods of carbapenemase-producing Pseudomonas aeruginosa Strains Isolated from intensive care units

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 497

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شناسه ملی سند علمی:

ICCM12_044

تاریخ نمایه سازی: 1 دی 1397

چکیده مقاله:

Introduction and Objectives: Carbapenems are one of the first-choice antibiotics for the treatment of Pseudomonas aeruginosa infections.but increase resistance to carbapenems in hospitals especially in intensive care units (ICUs) is a worldwide clinical concern. Resistance is mainly due to carbapenemases enzymes. Therefore, early and correct identify of carbapenemase-producing is important.,The present study was undertaken to detect carbapenemase in strains of P. aeruginosa isolated from ICU patient by using phenotypic and genotypic methods.Material and Methods :A total of 34 clinical specimens were collected from patients from November 2017 to May 2018in Hamedan,Iran.Antibiotic susceptibility tests were conducted by disc diffusion.Determination the minimum inhibitory concentration was done by E-test for imipenem antibiotic.To identify carbapenemases, combined disk tests (CDT) carbapenem inactivation method (CIM) carbaNP, Modified Hodge test (MHT) and genotypic methods were used.Results: During this study, 34 pseudomonas aeroginosa isolates were collected from ICU, between from isolates 15carbapenem-resistant pseudomonas aeroginosa isolates were subjected to CarbaNP ,MCIM,MHT,IMP/EDTA tests, and tested by PCR for blaKPC, blaVIM, blaOXA48, blaSPM, blaSIM, blaGIM, blaAMPC, blaIMP genes .14/15 (93/33%) isolates were positive for carbapenemase production by Carba NP,14/15(93/33%) by MCIM ,9/15 (60%) by using DDST and 6/15 (40%) by MHT tests. Carba NP and MCIM tests showed a high sensitivity, specificity, PPV and NPV compared to PCR in Pseudomonas aeroginosa isolates.Conclusions:Our results showed that carbaNPand carbapenem inactivation method (CIM) tests are highly sensitive ,specific, cheap and rapid tests for the detection of carbapenemase producing in P. aeruginosa isolates.

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نویسندگان

Masoumeh Beig

MSc student of Medical Microbiology, Student Research Committee, hamadan University of Medical Sciences ,Hamadan, Iran

MohammadReza Arabestani

Phd Department of Microbiology, Faculty of Medicine, Hamadan of medical sciences, Hamadan,Iran