The demolition of neural cell cancer by gold nanoparticle coated with Glutamate

BACKGROUND: At present, the cancer after cardiovascular disease is the second leading cause of death in the world. To reduce the burden of these diseases people s awareness and attitude and devise more effective drugs and new treatment methods is important. Many cancer cells contain a protein called epidermal growth factor receptor (EFGR) on its surface that mainly is not found in healthy cells in the human body. These researchers binding gold nanoparticles to an antibody EGFR (with anti EFGR) have been able to attach mentioned nanoparticles to cancer cells and cause specific destruction of cancer cells with gold nanoparticles. Therefore, the aim of this study was to investigate the mechanism of degradation of gold nanoparticle coated with Glutamate amino acids on PC12 category of cancer cell. METHODS and MATHERIALS: In this study, PC12 cell category was cultured in vitro and these cells divided into 7 groups and then 6 groups exposed for 48 hours to varying concentrations of gold nanoparticles (0.5, 2.5 and 5 μM gold nanoparticles, Gold nanoparticles coated with Glutamate) and a group without nanoparticle as the control. The effect of nanoparticle cytotoxicity was examined by using MTT and LDH release from the cells. The phase contrast microscopy was used to evaluate the cellular morphology. Finally, the level of ROS in the cells was measured by using Fluorescent probe of 2, 7 Dichloroisocyanurate fluorescein Diacetate. FINDINGS: The average diameter of the gold nanoparticles in the case of uncoated and coated with Glutamate amino acids, respectively 10 ± 0.2 and 28 ± 0.5 nm was calculated. The most damaging effect of the studied cells by micro-molar concentrations of 25 gold nanoparticles coated with Glutamate amino acid, which occurred at a rate of 30% compared to the group treated with bare gold nanoparticles was a statistically significant difference (p≤ 0/05). The results of this study indicate that the majority of apoptosis of cells incubated with uncoated and coated gold nanoparticles is as apoptosis. CONCLUSION: The results showed that gold nanoparticles coated with Glutamate decreased metabolic activity and increased membrane destruction of the cell, in comparison with control cells in a dose-dependent mode and increased ROS and apoptosis mechanisms in cancer cells causing cell death in PC12 category.

کلیدواژه ها:

cancer ، nanoparticle ، Glutamate ، apoptosis