The effect of crocin on MDA as a biomarkers of oxidative stress in the Saliva of multiple sclerosis patients; a randomized controlled clinical trial

Background and Objective: Multiple sclerosis (MS) is a chronic and progressive demyelination disorder in the central nervous system, the cause of which is still unknown. Inflammatory factors and oxidative stress play a very important role in the development of this disease. Saffron, as a potent and anti-inflammatory antioxidant, improves the oxidative stress indices, oxidative load damage and inflammation. Therefore, this study aimed to determine the effect of crocin on Malondialdehyde (MDA) concentration as biomarkers of oxidative stress in the saliva of patients with MS. Materials and Methods: A randomized controlled trial was conducted on patients with multiple sclerosis referred to Farshchian Hospital in Hamadan in 2017. Patients were randomly divided into two groups and assigned to a group of patients with a crocin capsule and to another group of placebo capsule for 4 weeks. After the treatment period, in the saliva samples, the amount of MDA was measured by spectrophotometer. Data analysis was conducted using SPSS 22 software and our IRCT number is: IRCT2016122013194N3. Results: Thiobarbitoric acid (TBA) assay is the most commonly used method for determination of the MDA. According to the TBA Results, MDA did not differ significantly in the control group between beginning and after receiving placebo (P =0.061). But, after 4 weeks treatment with crocin in the case group significant decrease of MDA was seen (P=0.016). Conclusion: The recognition of the pharmacokinetics of saffron and its components in the field of effective and positive effects on diseases is very important. Given that little studies have been done so far, animal and human studies in this field, especially on MS disease, are necessary.

کلیدواژه ها:

Crocin ، Oxidative Stress ، MDA ، Saliva ، Multiple sclerosis