Endothelial Cells Adhesion and Proliferation on Collagen/Hyaluronic Acid/ Bioglass Nanoparticles Scaffolds

Background and Aim: In recent years, many studies have focused on the synthesis of suitable scaffolds for wound healing with different structures. Among multiple three-dimensional scaffold fabrication techniques, the freeze-drying method can be used to create porous structures for inducing endothelial (HUVEC) cells adhesion and proliferation. The biological healing process is influenced by angiogenesis, microenvironment, and extracellular matrix. This evaluation aimed to study the effect of the collagen/hyaluronic acid/ bioglass nanoparticles scaffolds on endothelial cells behavior. Methods: Collagen/hyaluronic acid/ bioglass nanoparticles (BGNPs) (various ratios of BGNPs: 0.5 wt%, 1.5 wt% and 3 wt %) were fabricated and their biomechanical, physicochemical and biocompatibility properties were examined. The morphology of collagen/hyaluronic acid/BGNPs scaffolds were evaluated with scanning electron microscopy (SEM). MTT and DAPI nuclear staining were performed to assess cell adhesion and proliferation on freeze-drying samples. Results: Microscopic images and nuclear staining of cultured endothelial cells on collagen/hyaluronic acid/BGNPs scaffolds denoted the better adhesion and proliferation of HUVEC cells on the freeze-drying scaffolds with 1.5% BGNPs content. The results of biocompatibility analysis revealed that the most suitable ratio for endothelial cells proliferation was collagen 2% and 2.5% hyaluronic acid incorporated with 1.5% BGNPs. Conclusion: The collagen/hyaluronic acid/BGNPs scaffold in our study was shown to have a suitable impact on cellular viability. So, collagen/hyaluronic acid/BGNPs scaffolds could be a promising template to promote angiogenesis needed in the wound healing process.

کلیدواژه ها:

HUVEC ، Freeze-Drying Technique ، Collagen/Hyaluronic Acid/BGNPs