Cell Viability Induction by PVA/CMC Nanofiber to Wound Healing

Nowadays, tissue engineering extremely used for different healing types of the skin wound and skin tissue repair. In this study, full-thickness excisional wound healing is considered by tissue engineering methods. At first, nanofiber scaffolds prepared by electrospinning in different ratio of polyvinyl alcohol/ carboxy-methyl chitosan (2, 3, 4 and 5%) and is cross-linked by glutaraldehyde. Morphology of nano-fibers was studied by SEM and cell viability rate on the scaffolds evaluated by MTT assay using human fibroblasts (HDF) and human placental mesenchymal stem cells (PMSCs). Our results indicated that the PVA/CMC 4% scaffold could provide cell viability. It seems that this type of the scaffold can be used for wound healing also, can accelerate the healing range in full-thickness excisional wounds.

کلیدواژه ها:

Tissue Engineering ، Nanofiber ، Carboxy-Methyl Chitosan ، Skin Wound Healing