Human Amniotic Membrane Mesenchymal Stem Cells: A Revolution in Wound Healing

The mesenchymal stem cells (MSCs), which are derived from the mesoderm, are considered as a readily available source for tissue engineering. They have multipotent differentiation capacity and can be differentiated into various cell types. Many studies have demonstrated that the MSCs identified from amniotic membrane (AM-MSCs) are shows advantages for many reasons, including the possibility of noninvasive isolation, multipotency, self-renewal, low immunogenicity, anti-inflammatory and nontumorigenicity properties, and minimal ethical problem. The AM-MSCs may be appropriate sources of mesenchymal stem cells for regenerative medicine, as an alternative to embryonic stem cells (ESCs). Recently, regenerative treatments such as tissue engineering and cell transplantation have shown potential in clinical applications for types of wounds. Therefore, amnion and MSCs derived from amnion can be applied to cell therapy in wounds. We isolation and the phenotypic characterization of mesenchymal stem cells from the amniotic membrane. The placentas included in the study were derived from pregnancies with a normal evolution. Along with the placentas, umbilical cord blood and maternal peripheral blood samples were taken. The isolation and the culture of cells from the amniotic membrane was followed by the determination of the markers of these cells. The cells expressed markers characteristic of stem cells. Immunofluorescence evidenced the pluripotential properties of these cells.

کلیدواژه ها:

Amniotic Membrane ، Mesenchymal Stem Cells ، Wound Healing