Ultrasound-Assisted Emulsification Microextraction Coupled with HPLC-DAD for Determination Trace Level of Diclofenac in Urine and Water Samples

In this research, a simple, selectivity, and environmentally friendly method based on ultrasound-assisted emulsification microextraction (USAEME) was proposed for determination of trace level of diclofenac in urine and water samples. High performance liquid chromatography with diode-array detection (HPLC-DAD) was employed to analysis the extraxtants. Coupling the USAEME method with HPLC-DAD was presented for the first time in this study. Several analytical parameters affecting the USAEME method on the recovery extraction were investigated and optimized. The expermental conditions, including pH of sample solution, type of extraction solvent, temperature and time of ultransound, centrifugation time and ionic strength were considered and optimized. Under the optimal conditions, relative standard deviations (RSD) of the analysis less than 6% (n= 3) and detection limit was determined 4.01 ng mL-1 for diclofenac. Recoveries of both in human urine and synthesis samples were in the ranges of 53–78%. USAEME - HPLC-DAD was successfully applied for determination of trace levels of diclofenac in human urine and water samples.

کلیدواژه ها:

Ultrasound-assisted emulsification-microextraction ، Diclofenac ، HPLC-DAD