THE USE OF BLUE-GREEN ALGAE IN INCREASING THE EFFICIENCY OF THE TISSUE CULTURE SYSTEM IN DATE PALM Phoenix dactylifera L. CV. BARHEE

The present study was carried out in the Plant Tissue Culture Laboratory of Fadak Company in Al-Bahadriya district in the province of Abi Al-Khasib in Basra Governorate for the period 2015-2017. The aim of the study was to add the biomass extracts of Oscillatoria tenuis to the media for in vitro culture of date palm (Phoenix dactylifera L.) cv. Barhee to determine its effect on direct organogenesis. The treatment of the medium supplemented with the full strength of MS salts and 20% of the water extract of the biomass of blue-green algae resulted in a significant increase in the percentage of response to direct shoot proliferation and rooting of the shoot. The same treatment resulted in an increase in the number of shoots resulting from proliferation, primary and secondary adventitious roots, the fresh and dry weight of vegetative shoot and roots, leaf content of total chlorophyll, nitrogen, phosphorus and potassium. The treatment of the medium supplemented with the full strength of MS salts recorded the lowest values in all the traits mentioned above. The results of the study showed that the irrigation treatment of 20% of the water extract of the biomass of blue-green algae resulted in a significant increase in the plant survivor rate, plant height and number of leaves of the adapted plants, which reached 85.00%, 16.20 cm and 2.00 leaf /plant, respectively. The treatment of watering with distilled water gave the lowest mean percentage of the plant survivor rate, plant height and the number of leaves was 65.20%, 11.25 cm and 1.00 leaf /plant.

کلیدواژه ها:

Biomass extract ، Direct organogenesis ، MS salts ، Oscillatoria tenuis ، Proliferation