Effect of lead acetate exposure on spleen and small intestine of wistar rats histopathological findings

Effect of lead acetate exposure on spleen and small intestine of wistar rats histopathological findings Introduction and aims: Lead is an abundant metal in the Earth s crust and can be found naturally as a result of volcanic emissions and geochemical weathering. In Addition, its intense use in human activities has significantly increased its emission into the atmosphere. (1 in 19) A comparison of concentrations of lead in human tissues shows that Lead concentrations in bone greatly exceeded the concentrations in soft tissues and were highestin the dense bones. Bone lead concentrations increased with age in both sexes, more especially in male subjects and in dense bones.in other study in IV injection on lead acetate after 24hours the concentration of lead acetate in RBC cells was the highest and followed by liver,spleen,heart and lungs.in our life nowdays we are exposed into lead by different ways in air soils caused by different industries such as factories and Fossil Fuels. The aim is to evaluate the effect of lead acetate chronicexposure in small intestine and spleen of wistar rats. Material and methods: After acclimation period rats were devided into two groups 6 rats in each group the first group lead group was treated with 0.01% lead acetate in the drinking water in 30 days and the other group control group serving just distilled water At the end of the experimental period rats were sacrificed and the samples of spleen and small intestine were fixed in formalin and transferred to lab for histopathplogical evaluation. Result lead group:villous shortening (VS; blackarrow), variable degrees of fusion (VDF; whitearrow), epithelial atrophy (EA). These structural alterations of the intestinal mucosa (cell loss and altered crypt integrity) suggest that MTX induce pathomorphology, dysfunctions and structural changes in the small intestine (either change in subcellular, cellular, and histological structure).while these changes was not observed in control group Discussion: Lead is a cumulative poison and it affects every organ and system in the body2.Itaccumulates in bone, brain, muscle, liver, kidney, hematopoietic system, central nervous system and gastrointestinal system3,4,5(1258) Ingestion is the most common route of exposure to lead for children. Once lead has been swallowed, it enters a child’s body by absorption from the gastrointestinal tract. Children’s innate curiosity and their age-appropriate hand-to-mouth behaviour result in their bringing leadcontaining or leadcoated objects, such as contaminated soil or dust, to their mouth, and thus greatly increase their risk of exposure.(31)

کلیدواژه ها:

lead ، acetate ، spleen ، small intestine