Application of PCR in the Diagnosis of Neurocryptoccosis

Considering the low sensitivity of direct examination with India ink and culture to identifyCryptococcol meningitis is required to use of techniques sensitive.In this study, were used PCR for detection of Cryptococcus neoformans directly from CSFsamples to increase the diagnostic power in cases where conventional methods are incapable.In this cross - sectional study CSF samples of 25 patients with cerebral symptomsCryptococcol meningitis that was sent in Mycology Laboratory of Public Health TehranUniversity during the one year examined with India ink and culture and PCR. In secondphase produced two dilutions of 102 and 106 of Cryptococcus neoformans in 1ml CSF andwere compared three methods for direct examination, culture and PCR.Microscopic examination of samples with India was only one positive Also, both the dilution102 and 106 direct examination were positive. An example of the direct examination waspositive in culture also grew. Cultures of both dilution 102 and 106 were also positive. Insurvey With PCR examination only as a sample was positive in direct and culture also waspositive in terms of molecular. PCR examination of both dilution 102 and 106 were alsopositive.Samples that was positive in direct and culture also was positive in PCR examination.