Immunohistochemical detection of p53 and PCNA in ameloblastoma and adenomatoid odontogrnic tumor

Introduction:Although ameloblastoma and adenomatoid odontogenic tumor (AOT) belong to the samegroup according to the World Health Organization, they show different biologic behaviors.PCNA, an amplifier of cell proliferation, and P53, a tumor suppressor protein, areoverexpressed in some odontogenic lesions. the purpose of this was toimmunohistochemically evaluate the expression of p53 and PCNA to clarify the possible roleof these proteins in different behaviors of ameloblastoma and AOT.Material & method:The immunohistochemical expression of PCNA and p53 was determined in 30 solidameloblastomas and 12 AOTs. Statistical tests including one-way ANOVA, t-test, chi-square,Mann-Whitney U and Kendall were used to analyze the data .Results:All tissue sections (except one specimen of plexiform ameloblastoma) exhibitedimmunoexpression for p53. PCNA was expressed in all specimens. There was no significantdifference in PCNA expression between ameloblastomas and AOTs (P> 0.05) for p53, therewas no statistical difference between subtypes of ameloblastomas (P> 0.05) whereas statisticaldifferences were observed between ameloblastomas and AOTs (P<0.001) There was nostatistical difference in PCNA intensity of staining between aeloblastomas and AOTs(P<0.05) whereas the p53 intensity in ameloblastomas was stronger than AOTs. Positivecorrelation between PCNA and p53 was observed .Conclusion:We concluded that PCNA overexpression is not responsible for the difference in clinicalbehavior of these two lesions, whereas the expression of p53 in ameloblastoma may explainthe more aggressive nature of this tumor compared with AOT.

کلیدواژه ها:

Ameloblastoma ، adenomatoid odontogenic tumor ، PCNA ، P53 ، Immunohistochemistry