In Vitro Comparison of Chromosomal Radiosensitivity in Acute Lymphocytic Leukemia Patients and Common VariableImmunodeficiency

Background:Common variable immunodeficiency (CVID) is one of the predominantly antibodydeficiency disorders which increasing evidence suggests that dose-dependent chromosomaldefects are present in these patients.Objective:The aim of this study was to investigate the chromosome radiosensitivity in peripheral bloodlymphocytes of CVID patients and their first degree relatives and to compare the results witha group of acute lymphoblastic leukemia (ALL) patients and healthy individuals.Methods: The in vitro radiation sensitivity was measured with the G2 and the G0-micronucleus assays. The results in each group were also compared with ataxia telangiectasia(AT) cases as positive control.Results:By two methods of analysis (crude mean and cut off point), the CVID cases weresignificantly more radiosensitive than healthy control based on the results of the G2 as wellas the G0 assays. Patients with parental consanguinity are more prone to irradiation asmeasured by the frequency of chromatid breaks (P=0.008) and rearrangements (P=0.044).Surprisingly first degree relatives of CVIDs had similar radiosensitivity to CVID cases inchromatid breaks which showed significant difference with normal controls.Conclusions:Results indicate that most of the CVID patients and their first degree relatives are sensitive toionizing radiation similar to ALL cases. This study may support that chromosomeradiosensitivity in CVID patients could be a marker of genetic predisposition to the disease.These patients should be protected from unnecessary diagnostic and therapeutic proceduresusing ionizing radiation.

کلیدواژه ها:

Chromosome radiosensitivity ، Ataxia telangiectasia ، Common variable immunodeficiency ، Acute lymphoblastic leukemia