ANTIMICROBIAL EFFECT OF SILVER-NANOPARTICLES ON BACITRACIN-POLYMIXIN-GENTAMYCIN-CLINDAMYCIN AND ERYTHROMYCIN AGAINST BACILLUS SUBTILIS- BACILLUS CEREUS- PSEUDOMONAS AERUGINOSA- E-COLI

Background and Aim: Although the majority of infections are now curable, but their effectiveness have been blocked with progressing phenomenon of worldwide Antibiotic resistance among microbial pathogens. Probably, the best solution is to increase their effects by means of various Nanoparticles Methods: Bacterial spp. were prepared from National collection and cultured on standard bacteriological medium. The antimicrobial effects of AgNPs and Antibiotics against each strains were tested individually and the MIC were calculated. Finally, different combination ratio of each Antibiotic/AgNPs were prepared, and the activity of each combination were achieved against each bacterial spp. Triplicately and the data were analysed statistically. Results: The inhibition zone of AgNPs against E.coli, P.aeruginosa, B.subtilis and B.cereus were 23, 17, 10, 10 mm. The inhibition zone of Combination of 25% AgNPs: 75% Clindamycin against gram positive spp. Was above 33mm and the same ratio of AgNPs and Gentamycin for gram negative spp. Was above 20mm. Conclusion: According to the final results of current research project, we concluded that the ratio of 25% AgNPs with 75% Antibiotics had better Antibacterial activities against our research bacterial spp. rather than Antibiotics or AgNPs solus.

کلیدواژه ها:

AgNPs ، Antibiotics ، Antibiotc resistancy