THE EFFECT OF BACILLUS SUBTILIS AND BACILLUS COAGULANS SPORES AS PROBIOTIC ON SALMONELLA CONCENTRATION IN THE INTESTINE AND ORGANS

Background and Aim:Salmonella infection has become a great concern in recent decades due to the high prevalence and antibiotic resistance. So finding novel preventive methods can be helpful. Recently, uses of probiotics, especially spore-forming bacteria such as Bacillus spp. have been growing up.Methods:In this study 40 rats were randomly divided into two groups as follows: Group 1: Receiving water containing 5×107 spore/ml Bacillus subtilis and 5×107 spore/ml Bacillus coagulans for 29 days and intragastric gavage of 109 CFU of Salmonella Typhimurium at day 22. Group 2: Receiving intragastric gavage of 109 CFU of Salmonella Typhimurium at day 22. At day 23, 25, 27 and 29, five rats were chosen randomly from each group and Salmonella was counted in the liver, spleen, mesenteric lymph node, content of ileum and cecum and feces.Results:On the first day of sampling the number of Salmonella in the liver, spleen and mesenteric lymph node was zero. At day 25, 27 and 29 the Salmonella count had been reduced by up to 0.5, 0.8 and 1.4 log in the liver, 0.4, 0.6 and 0.7 log in the spleen and 0.4, 0.6 and 0.7 log in the mesenteric lymph node respectively. At day 23, 25, 27 and 29 the Salmonella count had been reduced by up to 0.9, 0.2, 0.1 and 0.7 log in the ileum, 0.4, 0.2, 0.3 and 0.7 log in the cecum and 0.4, 0.2, 0.1 and 0.7 log in the feces respectively.Conclusion:Spore-forming probiotics can be mentioned as a preventive method for Salmonella infection.

کلیدواژه ها:

Spore-forming probiotic ، Bacillus subtilis ، Bacillus coagulans ، Salmonella Typhimurium