EFFECT OF BIFIDOBACTERIUM BIFIDUM ON REDUCING BLOOD SUGAR AND CHOLESTEROLE IN DIABETIC RATS

Background and Aim:Diabetes mellitus is a disease which has affected about 3 million people in Iran. For many years, the use of Phytotherapy in medicine has been common. In the present study, the effects of native probiotic on blood glucose s in diabetic rats were investigated. Probiotics are living microorganisms that administration of sufficient quartiles of them cause health promotion. Since Probiotics modulate the composition of the intestinal microflora, they plays an important role in ameliorating the side effects of diabetes.Methods:: For this study, 21 male wistar rats aged 12-14 weeks and weighed 110-130 gm was used. After a 1-week adaptation period, the rats were randomly divided into three group positive control, negative control (diabetic), trail 1 (probiotic) . Diabetes was induced through the injection of stereptozotocin (55 mg/kg) into the experimental case and diabetic control . Trail1 group was administrated with 2×109 cells of bifidobacterium bifidum, by oral gavage . After 21 days, rats were anesthetized with ether and blood samples were taken from heart.Then Glucose kit is used for quantitative determination of Glucose in plasmaResults:Our results showed that Glucose level in positive group (268±6.106 mg/dl) was significantly increased compare to negative control(92.29±2.697 mg/dl), (Pvalue < 0.0001). It also became clear that receiving probiotics in first trial group caused a significant decrease in Glucose concentration to(124.6±1.478mg/dl), (Pvalue < 0.0001).Conclusion:The results of these tests indicate that the probiotic bifidobacterium bifidum as a probiotic can reduce the negative effects of diabet and reduce levels of Glucose blood significantly.

کلیدواژه ها:

Diabetes ، Probiotic ، glucose ، rat