INVESTIGATION OF THE PRESENCE OF BIOACTIVE PEPTIDES IN TARKHINEH AND THEIR ANTIOXIDANT ACTIVITY

Background and Aim:Bioactive peptides have been defined as specific protein fragments that have a positive impact on body functions or conditions and may ultimately influence health. Hydrolytic reactions, such as those catalyzed by digestive enzymes, result in their release. Tarkhineh is a prepared product that usually consists of cracked wheat, yogurt, and vegetables that are fermented. This product considered as probiotic and it is believed that Tarkhineh is the source of bioactive peptides, so the aim of this study was investigation of the presence of bioactive peptides in Tarkhineh and their antioxidant activity.Methods:Tarkhineh were made in laboratory scale and their aqueous extract were prepared. Protein content of these extracts measured by Bradford method. Extracts were ultra-filtrated with nominal molecular weight exclusion limit of 5000 and 10000Da and the antioxidant activity of these filtrates were investigated by DPPH method.Results:The protein content of extracts was 1.52 ± 6 mg/ml. The DPPH results showed that antioxidant activity of unfiltered extracts are significantly higher (p < 0.05) than the 5000 and 10000 Da fractions. In the other hand, filtrate by molecular weight exclusion limit of 5000 Da showed better antioxidant activity than 10000 Da fractions (p < 0.05).Conclusion:The results of this study suggested that the Tarkhineh can have bioactive peptides with antioxidant activity and therefore can have health effects on consumer.

کلیدواژه ها:

bioactive peptides ، Tarkhineh ، antioxidant activity ، DPPH