THE MORTALITY RATE OF EPSILON TOXIN CLOSTRIDIUM PERFRINGENS TYPE D
محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 374
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شناسه ملی سند علمی:
MEDISM19_680
تاریخ نمایه سازی: 13 مهر 1397
چکیده مقاله:
Background and Aim:Clostridium perfringens (C. perfringens) is classified into five types A to E, based on the secretion of four major toxins, alpha, beta, epsilon, and yota. C. perfringens type D, which secretes the main toxin of epsilon, causes enterotoxemia and mortality in domestic animals, so it is important to discuss for the health of livestock industry and also for providing the meat that is need of the community.Methods:In this research, the amount of epsilon toxin that released from C. perfringens type D isolated from livestock was investigated. At First, the C. perfringens bacterium type D that was confirmed by multiplex PCR technique cultured on the nutrient medium. After incubating in anaerobe condition and ensuring growth, the supernatant was removed and activated by the enzyme. Then dilutions prepared from 1.10 to 1.200. From each dilution 0.5 ml was injected to N.M.R.I mice intravenously.Results:The minimum lethal dose of epsilon toxin level was 75.Conclusion:The mortality rate obtained from our samples was the significant difference with standard strain.
کلیدواژه ها:
نویسندگان
Ali Reza Karimabadi
Department of Research and Development, Razi Vaccine and Serum Research Institute, Kerman Branch, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran
Mehrdad Shamsaddini Bafti
Department of Research and Development, Razi Vaccine and Serum Research Institute, Kerman Branch, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran