EFFECT OF PROTECTION VACCINE TS11 IN CILINICAL SAMPLES FROM COMMERCIAL AND DOMESTIC POULTRY WITH PCR ASSAYE

Background and Aim:Mycoplasmose a widespread infectious disease that has affected the poultry industry around the world Mycoplasma gallisepticum(MG) belongs to the Mollicutes class and is one of the smallest free living. micro organism that grow in a very nutritious environment. The aim of this study was to investigate and detect Mycoplasma gallisepticum from industrial and domestic broiler chickens in Urmia by polymerase chain reaction (pcr).Methods:The chickenpox of the suspected poultry was collected by sampling swab. After transferring samples to The bacteriological laboratory was attempting to extract DNA Using mycoplasma galli septicemia specific primers on all samples taken in the gyrb region, the PCR reaction done.Results:This study experiment randomized 50 samples of broiler commerical poultry with evident clinical manifestations of mycoplasma gallisepticum, 3samples with a specific primer designed, were positive PCR, and in 20 samples from domestic poultr, 6 cases were positive.Conclusion:The result of the TS11 vaccine is somewhat immune, and contamination in commercial poultry is lower infection than of domestice non-vaccinated individuals

کلیدواژه ها:

poultry ، Mycoplasma ، gallisepticum ، TS11