MOLECULAR CHARACTERIZATION OF THE CHICKEN ANAEMIA VIRUSES ISOLATED FROM BROILER FARMS OF EAST AZERBAIJAN, IRAN

Background and Aim:Infectious anemia of poultry as one of the immunosuppressive viral diseases is associated with high mortality and economic losses worldwide. The causative agent of the disease, CAV (Chicken Anemia Virus), is a non-enveloped, icosahedral virus with a negative-sense, single-stranded circular DNA genome. In the present study, molecular analysis of CAV circulating isolates from East-Azerbaijan, Iran was done.Methods:100 Liver samples from broilers were collected. DNA extraction of the samples were done and then, amplification of the VP1 gene was carried out. PCR products were purified and finally, the purified products were subjected to restriction fragment length polymorphism (RFLP) using XbaI and AluI endonuclease enzymes digestion.Results:VP1 gene were amplified in 38 of 100 obtained samples successfully. Viral isolates were classified to 10 different groups using AluI while digestion with XbaI were grouped the virus isolates in seven distinct clusters.Conclusion:Results of the current study suggests high rate of genetic variation among virus circulating isolates of CAV in poultry farms of East Azerbaijan of Iran.

کلیدواژه ها:

CAV-anemia-Vp1