DETERMIND VIRULENCE FACTORAND ANTIBIOTICS RESISTANCE AMONG CLINICAL ISOLATES OF UROPATHOGENIC E.COLI IN NORTHEAST OF IRAN
محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 433
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شناسه ملی سند علمی:
MEDISM19_592
تاریخ نمایه سازی: 13 مهر 1397
چکیده مقاله:
Background and Aim:Urinary tract infections (UTIs) are one of the most common infectious diseases. Although different bacteria can be responsible for UTIs. E.coli is the most frequently isolated pathogen in UTIs. The pathogenic potential of E. coli strains dependent on the presence of virulence factors. The aim of this study was to determine the presence of main virulence genes by multiplex PCR and determine antibiotics resistance among clinical isolates in northeast of Iran.Methods:three hundred Escherichia coli isolates obtained from patients with urinary tract infection (UTI) referred to the Ghaem and Emam Reza Hospital different section, Mashhad, Iran. A Multiplex polymerase chain reaction (PCR) was used for the amplification of genes encoding pyelonephritis associated pili (pap genes), S-family adhesions (sfa gene), type1fimbriae (fimH gene), aerobactin (aer gene). The antibiotic susceptibility tests were done by the disk diffusion method. Different antibiotics were used such as B lactam, Aminoglycosides, Cephlosporine, Quinolone, Flurquinolone, Carbapenem and Trimethoprim-sulfamethoxazole.Results:The PCR results identified the fimH gene in (78.4%), aer in (70.5%), sfa gene in (13.6%) and the pap gene in (8.2%) of the isolates. The rate of antibiotic resistance of the strains is as follows: 64.7% resistant to cephalosporins, 34% to trimethoprim-sulfamethoxazole, 30.9% to fluoroquinolones, 15.2% to aminoglycosides, 13.2% to beta lactams 7.8% to quinolones and 4.4%. to carpabenems.Conclusion:in these study fimH and aer were found more than 50% UPEC strain isolated from patients that two gene have important role in pathogenesis. Major isolates have fimH as adhesion factor for colonization. More isolates resistance to cephalosporins cause of inappropriate administration.
کلیدواژه ها:
نویسندگان
Mahdis Ghavidel
Microbiology and Virology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Microbiology and virology, Mashhad University of Medical Sciences, Mashhad, Iran
Tahereh Golamhosseini Moghaddam
school of veterinary medicine, shiraz , Iran
Kimia Nourian
DVM, graduate student of school of veterinary medicine, Ferdowsi university of Mashhad, Mashhad, Iran
Kiarash Ghazvini
Microbiology and Virology Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran. ۲. Department of Microbiology and virology, Mashhad University of Medical Sciences, Mashhad, Iran