DETECTION OF TETRACYCLINE AND FLUOROQUINOLONES RESISTANCE AMONG CAMPYLOBACTERS ISOLATED FROM DIARRHEA PATIENTS IN MARKAZI PROVINCE - 2015

Background and Aim:Campylobacter species, especially C. jejuni and C. coli, are the most common bacterial causes of gastroenteritis in humans.In recent years, tetracycline and fluoroquinolones resistance have emerged among many pathogenic and nonpathogenic species of bacteria .In Iran, limited studies have been determined antibiotic resistance of these bacteria,therefore, this study aimed to determine the frequency of tetracycline and fluoroquinolones resistance and to investigate genotypic determinant of tet(O) and gyrA resistance in campylobacters isolated from the stools of the dysenteric patients admitted to educational and medical centers in Arak, IranMethods:In this descriptive cross-sectional study, diarrheal stool specimens of the patients admitted to educational and medical centers in Arak in the period from May 2015 to Sep. 2015 were collected and after phenotypic and genotypic investigation of campylobacter, resistance to tetracycline and fluoroquinolones were assessed using disk diffusion method. In addition,tet(O) and gyrA genes contributing to tetracycline and fluoroquinolones resistance in campylobacter were also investigatedResults:Out of 108 dysenteric stool specimens, 28 (26%) campylobacter were isolated of which 26 (24%) were C. jejuni and 2 (1.8%) were C. coli. Phenotypic tetracycline and fluoroquinolones resistance prevalence in C. jejuni was obtained as 19 (73%),16 (61.5%) and in C. coli as 2 (7.1%) of all 2 types of resistance respectively.Frequency of,tet(O) tetracycline and gyrA fluoroquinolones resistance gene was found as 17 (65.3%),14 (53.8%) and 1(50%),1(50%) in C. jejuni and C. coli, respectivelyConclusion:This study showed tetracycline and fluoroquinolones resistance in campylobacter isolates. This is very useful data for empirical therapy and demanding comprehensive study with more samples size to be accomplished.

کلیدواژه ها:

Campylobacter ، tetracycline ، fluoroquinolones ، Drug Resistance ، Bacterial ، Iran