MOLECULAR ANALYSIS OF PSEUDOMONAS AERUGINOSA ISOLATED FROM CLINICAL, ENVIRONMENTAL AND COCKROACH SOURCES BY ERIC-PCR

Background and Aim:The objective of this study was to investigate the antibiotic susceptibility, virulence factors and clonal relationship among P. aeruginosa isolated from environmental sources, hospitalized patients and the surfaces of cockroaches in ICUs of four hospitals in Hamadan, west of Iran.Methods:A total of 237, 286 and 156 bacterial isolates were collected from clinical, environmental and cockroaches, respectively from May to September, 2017. The antimicrobial susceptibility determined using the disk diffusion method. The virulence factors; exotoxins A, S and U were detected by PCR. The genetic linkage of P. aeruginosa isolates were analyzed by Enterobacterial Repetitive Intergenic Consensus (ERIC) - PCRResults:According to our findings, 58 (24.4%), 46 (16%) and 5 (3.25) P. aeruginosa was isolated from clinical, environmental and cockroaches samples, respectively. The MDR phenotypes were detected in 18 (45%) and 15 (37.5%) of Clinical and environmental strains. The environmental isolates harbored more exotoxin A and exoenzyme S than clinical isolates. A genetic diversity was established among P. aeruginosa isolates, which 14 different ERIC fingerprints were detected. The clonal relationship was detected among clinical, environmental and isolates from cockroachesConclusion:Our results indicated to the importance identification and controlling the potential sources of P. aeruginosa infections in hospitals

کلیدواژه ها:

Pseudomonas aeruginosa ، ERIC-PCR ، Clinical ، environmental ، Cockroaches