ANTIBIOTIC RESISTANCE GENES CODING OF EFFLUX PUMP (ADEA AND ADES GENES) IN ACINETOBACTER BAUMANNII ISOLATED FROM PATIENTS BY MOLECULAR METHODS

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 537

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شناسه ملی سند علمی:

MEDISM19_577

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Background and objective: One of the important mechanisms of resistance in this bacterium is multidrug efflux pumps, playing an essential role in development of resistance in this bacteria. The aim of this study has been investigating the frequency of adeA and adeS genes of adeABC efflux pump in Acinetobacter baumannii clinical isolates.Methods:Materials and methods: 60 Acinetobacter baumannii isolates were collected from different hospitals in Tehran, and detected using standard biochemical tests. Eventually, they were confirmed by detecting blaOXA-51-like gene using PCR method. The pattern of antibiotic sensitivity was determined by agar disk diffusion method according to CLSI instructions. adeA and adeS genes of adeABC efflux pump were detected in the isolates using PCR method.Results:Results: the frequency of resistance to antibiotics in Acinetobacter baumannii isolates was as follows: piperacillin 100%, ceftazidime 98%, amikacin 96.66%, tetracycline 92%, ampicillin-sulbactam 65%, meropenem 63.33%, ciprofloxacin 60%, imipenem 50%, and gentamicin 48.33%. The frequency of presence of adeA and adeS genes in the Acinetobacter baumannii isolates was 80% and 81.66%, respectively.Conclusion:Conclusion: the high frequency of adeABC efflux pump genes as well as multidrug resistance across all of the studied Acinetobacter baumannii isolates suggests the fact that adeABC efflux pump is one of the mechanisms for development of drug resistance among these isolates to antibiotics.

نویسندگان

Mohammad Niakan

Shahed University.Medicine Faculty.Microbiology Department

Batool Basatian

MSc in Microbiology. Shahed University.Medicine Faculty

Zahra Soltani Kermanshahi

MSc in Microbiology. Azad islamic University.Science Faculty