DIAGNOSTIC OF VBNC BACTERIA IN SYNOVIAL FLUID OF RHEUMATOID ARTHRITIS PATIENTS

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 437

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شناسه ملی سند علمی:

MEDISM19_570

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:In recent years, the Viable but Non- Culturable (VBNC) of bacteria has created a global problem. Because of they are able to retrieve their metabolic activities and duration of the infectious disease without detectable. The aim of this study was set up diagnostic procedure of VBNC bacteria.Methods:In this study, a molecular based PCR method for recognition of VBNC was designed. Then, 50 synovial fluid samples which were stored in -80 ° C were evaluated. The bacteria genome extraction will be the first step, and identification of the presence of the bacterial genome by using the Universal primer (16srRNA) performed. Then, sequencing on amplicons was carried out and the results were analyzed. In the next step, the Nested PCR performed and to confirm the presence of the specific gene multiplication was determined. The sequencing of PCR products and BLAST alignment analysis were done.Results:The results revealed that, Of the 50 synovial fluid samples which were negative in bacteriological culture, 19 samples were positive in PCR base on 16srRNA gene. And Nested PCR had clarified of the 10 for Staphylococcus aureus strains as VBNC.Conclusion:However, all samples were negative for bacterial growth, the manifestation of Staphylococcus aureus was determined by nested PCR in the synovial fluid patients with rheumatoid arthritis. It may be illustrated the presence of the VBNC bacteria.

کلیدواژه ها:

VBNC ، Diagnostic ، Molecular Method ، Rheumatoid arthritis and synovial fluid

نویسندگان

Seyyed Reza Hashemi Miyan Abad

Department of Microbiology, Faculty of medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran

Ramezan Ali Ataee

Department of Microbiology, Faculty of medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran

Gholam Hossein Alishiri

Department of Rheumatology, Faculty of medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran

Mahdi Ghorbanali Zadegan

Department of Microbiology, Faculty of medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran