DETECTION OF SOME ADHESIVE GENES IN ESCHERICHIA COLI ISOLATES FROM PATIENTS WITH URINARY TRACT INFECTIONS IN MASHHAD BY MULTIPLEX PCR

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 357

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شناسه ملی سند علمی:

MEDISM19_569

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Urinary tract infection is the most common hospital infection that caused by colonization of uropathogenic E. coli in host mucosal epithelium and (infected)host tissue. Three virulence genes of this organism (adhesines) pap, sfa, afa are responsible for pathogenicity and adherence to epithelial cell. This study aimed to determine the prevalence of virulence genes in E. coli strains isolated from urine samples in Mashhad. The results of this study can determine information about the study area.Methods:In this study were collected 70 isolates of E. coli from outpatients with urinary tract infection and 45 hospitalized patients with Ute IN Ghaem hospital. Biochemical and standard microbiological techniques were used to identify the E.coli. . DNA extraction from samples was performed using the boiling method followed by gene Multiplex PCR method.Results:The prevalence of adhesines genes in hospitalized patients sample sfa 24.6% afa 21.5% pap 21.5%and in Outpatients samples are sfa 52.8% afa15.7% pap67.1%.Conclusion:The present study showed that the pap virulence genes are highly prevalent in outpatient’s samples and sfa genes are highly prevalent in hospitalized patients samples. The prevalent of sfa and pap is equal.

نویسندگان

Mahnaz Najafi

Master of Science in Microbiology

Mehrnaz Rad

DVM, Ph.D. Associated professor, Department of pathobiology.School of Veternaty Medicin Ferdowsi University of Mashhad

Gholamreza Hashemitabar

DVM, Ph.D professor, Department of pathobiology.School of Veternaty Medicin Ferdowsi University of Mashhad