ISOLATION AND MOLECULAR IDENTIFICATION OF BIOFILM PRODUCING PSEUDOMONAS AERUGINOSA BACTERIA FROM LIQUID STORAGE OF CONTACT LENS AND COSMETIC IN JAHROM PROVINCE

Background and Aim: Psuodomonas aeruginosa is known as the most prevalent agent of keratitis associated with contact lenses and the common organism cultured in eye trauma associated with non-contact lenses which leads to keratitis. The present study was done aiming isolation and molecular identification of Psuodomonas aeruginosa bacteria producing biofilm from preservation liquids of medical and cosmetic contact lenses in Jahrom city.Methods:The numbers of 324 samples were collected from preservation liquid of medical and cosmetic contact lenses from medical centers and students dormitories of Jahrom city during March to October of 2017. Psuodomonasbacteria were isolated and identified using phenotypic and molecular methods. Phenotypically, Disk Diffusion technique was carried out to determine the antibiotic resistant pattern and after DNA extraction, PCR technique was used to survey the biofilm production genes (PSLA).Results:Out of 324 samples, 28 samples were infected by Psudomonas. The maximum antibiotic resistant was observed in Nalidicsic Acid antibiotic (86%) and the maximum sensitivity was seen in Meropenem antibiotic (98%). Molecular results showed that 8 samples had PSLA genes.Conclusion:Our results clearly revealed the presence of biofilm producing Psuodomonasbacteria in the preservation solutions of contact lenses. Hygiene observation regarding the use and preservation of these lenses could be helpful in prevention of infection.

کلیدواژه ها:

Psuodomonas ، biofilm ، cosmetic and medical contact lenses