ECOR PHYLOTYPING AND DETERMINATION OF VIRULENCE GENES IN ESCHERICHIA COLI ISOLATES FROM PATHOLOGICAL CONDITIONS OF BROILER CHICKENS IN POULTRY SLAUGHTERHOUSES OF SOUTHEAST OF IRAN

Background and Aim:A number of virulence are considered important in the pathogenicity of these diseases. The APEC isolates are also assigned to different phylogenetic groups. The aims of the present study were phylogenetic typing and detection of virulence genes in Escherichia coli isolates from colibacillosis and cellulitis cases of broiler chicken in poultry slaughterhouses of Shahrbabak region, Kerman, IranMethods:A total number of eighty three E. coli isolates were taken from broiler chickens with colibacillosis and thirty four isolates were taken from carcasses with cellulitis in the industrial slaughter houses. E. coli isolates were confirmed biochemicallyResults: The confirmed E. coli isolates were subjected to PCR assays to determine phylogenetic groups and the presence of pap C, sfa/focDE, iucD, afaIB-C, hlyA, fimH and crl virulence genes. Colibacillosis E. coli isolates belonged to A (54.21%), B1 (7.22 %), B2 (6.02 %) and D (32.53 %) phylogroups. Whereas, the E. coli isolates from cellulitis cases belonged to three main phylogroups A (55.88%), B1 (5.88%) and D (38.23%). Statistical analysis showed a specific association between the presence of crl virulence gene and phylogroups of A and D (P < 0.05) in colibacillosis isolates. According to the resultsConclusion:E. coli isolates from colibacillosis and cellulitis broilers could be assigned to various mainly A, phylogenetic groups

کلیدواژه ها:

Escherichia coli ، Phylogenetic group ، Celluitis ، Collibacillosis ، Virulence genes ، Broiler chicken