OPRL CLONING OF PSEUDOMONAS AERUGINOSA IN ESCHERICHIA COLI BL21 AFTER BIOINFORMATICS VERIFICATION AS A CANDIDATE FOR VACCINE.
محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 470
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شناسه ملی سند علمی:
MEDISM19_545
تاریخ نمایه سازی: 13 مهر 1397
چکیده مقاله:
Background and Aim:Pseudomonas aeruginosa is a Gram-negative bacterium that is considered to be one of the most important bacterial pathogens responsible for serious opportunistic infections among cystic fibrosis (CF) and immunocompromized patients. Perhaps, choosing the right vaccine can solve the problem. So, we suggest other membrane protein L (OprL).Methods:Primer design, PCR (Polymerase Chain Reaction) and cloning for oprL in the plasmid pET 28a done after bioinformatics studies. Then, its transfer to Escherichia coli BL21 was performed. Finally, it was approved by universal primer PCR and enzymatic digestion methods.Results:The bioinformatics studies shown protein conservation. Also, it was strong antigenic and immunogenic properties for OprL by IEDB Analysis and vaxijen software respectively. In the laboratory experiments, the oprL was cloned in Escherichia coli BL21. Finally, the approval tests were positive.Conclusion:This protein is suitable for evaluating animal experiments.
کلیدواژه ها:
نویسندگان
Omid Shirmohammadi
Department of Microbiology, Hidaj Branch, Islamic Azad University,Hidaj, Iran.( Student of MSc microbiology)
Mohammad Hadi Fakoor
Department of Microbiology, Hidaj Branch, Islamic Azad University, Hidaj, Iran. (faculty member of Islamic Azad University)