EVALUATION OF HERPES SIMPLEX VIRUS I&II IN SUSCEPTIBLE PSEUDOMONAS AERUGINOSA KERATITS BY PCR MOLECULAR METHOD

Background and Aim:Herpes simplex virus (HSV) causes a wide range of diseases in humans. The main idea in this study is to evaluate the presence of HSV I&II in clinical susceptible pseudomonas keratitis. To investigate the presence of HSV virus in susceptible pseudomonas aeruginosa keratitis by PCR molecular methodMethods:70 samples of suspected Pseudomonas keratitis were collected from the eye by a specialist physician from Labafinejad Hospital. DNA extraction from samples was done by boilingDNG plus method. The PCR test was optimized and specificity and limit of detection (LOD) were performed on samples.Results:Amplicon with the size of 454bp observed in 1.5% agarose gel electrophoresis. Out of 70 samples, 5 cases (7%) were positive for HSV. Primers were banded just with HSV DNA in specificity test, that indicating high specificity. The LOD was indicated 50 copy/reactionConclusion:Certainly, some of the detected pseudomonads keratitis is clinically related to the presence of HSV, which is further investigated. In addition, the PCR molecular method is a fast, precise, high-precision method for detecting factors.

کلیدواژه ها:

Herpes Simplex Virus ، keratitis ، PCR ، Pseudomonas aeruginosa