PREVALENCE OF THE CLOSTRIDIUM DIFFICILE TOXINS IN TEHRAN HOSPITALS
محل انتشار: نوزدهمین کنگره بین المللی میکروب شناسی ایران
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 328
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شناسه ملی سند علمی:
MEDISM19_530
تاریخ نمایه سازی: 13 مهر 1397
چکیده مقاله:
Background and Aim:Introduction and Objective: Clostridium difficile (C. difficile) is an important nosocomial pathogen in hospitals and causes antibiotic-associated diarrhea and pseudomembranous colitis. The main virulence factors of C. difficile include the toxin A (TcdA), toxin B (TcdB) and binary toxin (CDT), produced by some isolates of C. difficile. The role of CDT during infection has not been well understood, but it has been elucidated that CDT enhances virulence conferred by toxin A and B in animal models.Methods:Materials and Methods: A total of 580 stool samples were collected from patient with suspected to C. difficile infection (CDI). C. difficile was identified by culture onto enriched media and in anaerobic conditions. (PCR) was performed for detection of toxin A, toxin B, Cdta and Cdtb .Results: Results: A total of 60 (10 % of stool cultures) C. difficile were isolated from patients suspected to CDI. Of them, 7.3% (n=3) were positive for binary toxin, 87.7% (n=36) were positive for toxins A and B (A+B+), 7.3% (n=3) were positive for toxin (A-B+), 4.8% (n=2) for A+B- and 32% (n=19) were non-toxigenic C. difficile.Conclusion:Conclusion: Our data showed that existence of toxigenic (A+B+) C. difficile was high in the patients with long-term hospitalization, old age and use of previous antimicrobial drugs. Prevalence of binary toxin was relatively low and was found mostly in those patients with severe illness. Its production enhances virulence of toxin A&B.
کلیدواژه ها:
نویسندگان
Fatemeh Savaheli Moghadam
Departement of Bacteriology ,Faculty of Medical Sciences ,Tarbiat Modares University ,Tehran ,IRAN
Ashraf Mohabati Mobarez
Departement of Bacteriology,,Faculty of Medical Sciences,Tarbiat Modares University,Tehran,Iran
Sepideh Khodaparast
Departement of Bacteriology,,Faculty of Medical Sciences,Tarbiat Modares University, Tehran.Iran