THE EFFECT OF UV MUTAGENESIS TO PRODUCE MORE CLAVULANIC ACID BY FACTORS SUCH AS THE PRODUCTION OF ENZYMES, THE CONSUMPTION OF CARBON AND NITROGEN, AND ENZYMES AND SUGARS. STREPTOMYCES CLAVULIGERUS

Background and Aim:In this research, a method of mutagenicity with ultraviolet radiation was used to increase the ability of clavulanic acid production by S. clavuligerus. To the results, 16 strains were eliminated and only 6 strains were identified as the highest total production activity clavulanic acid was selected, this strain again in the fermentation culture and HPLC Method for the production of antibiotics than the strain DSMZ 738 was determined and then in the second round of mutagenesis to increase the production capability of the strain in the process of mutagenesis Ultraviolet radiation was used and after culture in a liquid medium, clavulanic acid production was measured by HPLC and results obtained were comparable to that of 738 DSMZ. To find out the relationship between the production of clavulanic acid with factors such as the production of enzymes, carbon and nitrogen and enzyme and sugar sources, standard one-way yields and six mutated strains were calculated.Methods:HPLCResults:The results show that mutagenesis has been responsible for the expression of some of the decomposing genes of the decomposition and inhibition of some of the genes.Conclusion:There is a link between the production of clavulanic acid with factors such as the production of enzymes, the consumption of carbon and nitrogen, and enzymes and sugars, a standard one-way yield, and six mutated strains.

کلیدواژه ها:

actinomycetes ، antibiotics ، clavulanic acid ، mutagenesis ، Streptomyces clavuligerus