DETECTION OF BIOFILM DEVELOPMENT IN CLINICAL ENTEROCOCCUS FAECIUM AND ENTEROCOCCUS FAECALIS ISOLATES FROM KASHAN

Background and Aim:Enterococcus faecium and Enterococcus faecalis are the most common Enterococci species, which are the most important cause of human enterococcal infections. The role of bacterial biofilms in repeated infections and antimicrobial resistance has huge significance for public health. The objective was to detection of biofilm development in clinical Enterococcus faecium and Enterococcus faecalis isolates from KashanMethods:A total of 48 isolates of Enterococci were collected from patients. Enterococcus species were identified using gram staining, grow in 6.5% NaCl and bile, hydrolyze esculin and others conventional microbiological methods. Quantitative determination of biofilm production was performed by using a modified Microtiter plate method.Results:Among the Enterococci species strains 36 (75%) isolates were found to be strong and 11 (22.92%) isolates 0% were found to be moderate and 1 (2.08%) isolates were found weakly adherent.Conclusion:The study demonstrates a high propensity among the isolates of Enterococcus faecium and Enterococcus faecalis to form biofilm which this is production biofilm can increase increases virulence of this bacterium.

کلیدواژه ها:

Enterococcus faecium ، Enterococcus faecalis ، Biofilm formation ، Method