IDENTIFICATION AND EXTRACTION OF ANTICANCEROUS ENZYMES FROM E.COLI AND A NEW METHOD TO STUDY ITS ACTIVITY

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 393

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شناسه ملی سند علمی:

MEDISM19_519

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:L-asparaginase is the most promising anti-tumour enzyme that reduces the level of L-asparagine (an important nutrient for cancer cells) resulting in cancer cell starvation which leads to the cell death. There are many sources of asparaginase but bacterial source i.e. E.coli and Erwinia spp is mostly use as a therapeutic agent against leukaemia.Methods:In this study screening of different bacteria was done by rapid plate assay for asparaginase producing capability then intracellular and extracellular Asparaginase were extracted from its potent producer i.e. E.coli which was isolated from sewage water. The activity of enzyme was determined by using new procedure i.e. rapid activity analysis on agar plate and in tubes.Results:Our results shows that an intracellular asparaginase enzyme was found to be more active against asparagines as compare to the extracellular enzyme. This enzyme isn’t only the requirement of therapy for tumour cells but also it is use in food industry so in upcoming years the demand for asparaginase will get increase and our new method for intracellular enzyme extraction and activity analysis method (quantitative and qualitative method) will gain its importance for being easy, less expensive, using less volume of enzymatic extracts.Conclusion:Our aim was the screening, production, extraction of LAsparaginase from E.coli and to develop a new and rapid procedure to analyze activity of the Asparaginase rather than using Nesslerization technique. we have found that Pseudomonas spp , E.coli and Staph spp were the asparaginase producers we have selected E.coli to be use in production and extraction steps.

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نویسندگان

Melika Farzin

medicine faculty shahid beheshti university of medical sciences